SITE-100
  • SITE100
    • PROJECT OVERVIEW
    • PROJECT AIMS
  • METHODOLOGY
    • General workflow
    • Field protocols >
      • BUILDING A FLIGHT INTERCEPTION TRAP
    • Specimen processing
    • Soil extractions
  • STUDY SITES
    • Oriental >
      • MIZORAM STATE, INDIA
      • HONGKONG
      • THAILAND
      • BORNEO (DANUM VALLEY), SABAH, MALAYSIA
      • GAOLIGONG MOUNTAINS
    • Palaearctic >
      • QINGLING MOUNTAINS, CHINA
      • BOOKHAM COMMON, UK
      • EUROPEAN ISLANDS (IBIOGEN PROJECT)
      • SPAIN
      • SOUTHERN URALS
      • TIANMU MOUNTAINS
      • GUTIAN MOUNTAINS
    • Americas >
      • PANAMA: CERRO HOYA
      • SOUTHERN APPALACHIANS, USA
      • PANAMA: BARRO COLORADO ISLAND
      • PANAMA: SANTA FE
      • HONDURAS
      • HUATULCO, MEXICO
      • CHAMELA, MEXICO
      • FRENCH GUIANA
  • SITE100 SERVER
  • GET INVOLVED
    • Adding an observatory
    • Soil observatories
    • Local barcoding studies
    • Naturalists and community science
    • PhD students and postdocs
  • CONTACT
  • New Page
Picture

Qingling Mountains, China

The Qinling Mountains, Shaanxi Province, are best known for the Panda preservation programs but also harbour a great diversity of insects, which have a long history of study.  An extensive sampling campaign was conducted by the Chinese Academy of Sciences under the direction of Dr Nie Ruie in the summer of 2016.
Picture
Picture
Site name:
  • FengXian (1512 – 1785 m a.s.l.; 34.21 – 34.18 N; 106.902 – 106.915 E;
  • Foping (1468 – 1538 m a.s.l; 33°40’ N; 107°58’ E;
  • MeiXian (1118-1309 m a.s.l., 34°4’52N;107°42′ E,
  • Zhouzhi (1350- 1564 m, 33°49′ N;108°15′ E

Project lead: RuiE Nie, Chinese Academy of Sciences

Status of project:

Sampling at the four main sites consisted of 10 plots each, sampled with FIT, Malaise traps,  10 pitfall traps, and beating of vegetation (three weeks)
(a) Number of DNA grade specimens (Coleoptera only)

Feng Xian: 12462

Fo Ping: 7756

Mei Xian: 9918

Zhouzhi: 9614
(b) Reference specimen set:

1248 specimens each representing separate ‘morphospecies’ (DNA extraction and images)
(c) DNA extractions: number of extractions, single or bulk

Bulk samples 1292 tubes of pools of either 50 or 25 specimens each
(d) DNA sequencing: genes sequenced, sequencing methodology

COI barcode/ metabarcode and shotgun (pooled morphospecies)

Sequencing methodology: Illumina sequencing platform_Hiseq 2500
(e) metabarcoding: marker used, number of samples

COI: primers: F:CCNGAYATRGCNTTYCCNCG&R: TANACYTCNGGRTGNCCRAARAAYCA with different tags
(f) Sequence data:  (Genbank accession xx-xx)

Not available

Picture

Navigation

Home
Methodology
Study Sites
SITE100 Server
Get Involved

Support

Contact
© COPYRIGHT 2019 SITE100. ALL RIGHTS RESERVED.
  • SITE100
    • PROJECT OVERVIEW
    • PROJECT AIMS
  • METHODOLOGY
    • General workflow
    • Field protocols >
      • BUILDING A FLIGHT INTERCEPTION TRAP
    • Specimen processing
    • Soil extractions
  • STUDY SITES
    • Oriental >
      • MIZORAM STATE, INDIA
      • HONGKONG
      • THAILAND
      • BORNEO (DANUM VALLEY), SABAH, MALAYSIA
      • GAOLIGONG MOUNTAINS
    • Palaearctic >
      • QINGLING MOUNTAINS, CHINA
      • BOOKHAM COMMON, UK
      • EUROPEAN ISLANDS (IBIOGEN PROJECT)
      • SPAIN
      • SOUTHERN URALS
      • TIANMU MOUNTAINS
      • GUTIAN MOUNTAINS
    • Americas >
      • PANAMA: CERRO HOYA
      • SOUTHERN APPALACHIANS, USA
      • PANAMA: BARRO COLORADO ISLAND
      • PANAMA: SANTA FE
      • HONDURAS
      • HUATULCO, MEXICO
      • CHAMELA, MEXICO
      • FRENCH GUIANA
  • SITE100 SERVER
  • GET INVOLVED
    • Adding an observatory
    • Soil observatories
    • Local barcoding studies
    • Naturalists and community science
    • PhD students and postdocs
  • CONTACT
  • New Page