SITE-100
  • SITE100
    • PROJECT OVERVIEW
    • PROJECT AIMS
  • METHODOLOGY
    • General workflow
    • Field protocols >
      • BUILDING A FLIGHT INTERCEPTION TRAP
    • Specimen processing
    • Soil extractions
  • STUDY SITES
    • Oriental >
      • MIZORAM STATE, INDIA
      • HONGKONG
      • THAILAND
      • BORNEO (DANUM VALLEY), SABAH, MALAYSIA
      • GAOLIGONG MOUNTAINS
    • Palaearctic >
      • QINGLING MOUNTAINS, CHINA
      • BOOKHAM COMMON, UK
      • EUROPEAN ISLANDS (IBIOGEN PROJECT)
      • SPAIN
      • SOUTHERN URALS
      • TIANMU MOUNTAINS
      • GUTIAN MOUNTAINS
    • Americas >
      • PANAMA: CERRO HOYA
      • SOUTHERN APPALACHIANS, USA
      • PANAMA: BARRO COLORADO ISLAND
      • PANAMA: SANTA FE
      • HONDURAS
      • HUATULCO, MEXICO
      • CHAMELA, MEXICO
      • FRENCH GUIANA
  • SITE100 SERVER
  • GET INVOLVED
    • Adding an observatory
    • Soil observatories
    • Local barcoding studies
    • Naturalists and community science
    • PhD students and postdocs
  • CONTACT
  • New Page
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SPECIMEN PROCESSING

Mass-trapped arthropods can be processed directly or pre-sorted by size or taxon. In all cases the SITE100 protocol aims to preserve the specimens slated for DNA extraction as much as possible, either by non-destructive DNA extraction or by imaging the specimens prior to processing. We prefer a photographic record wherever possible. The procedure involves the following steps:
  1. All specimens are photographed individually or in bulk (<50 specimens per image), possibly after size sorting and taxonomic sorting (e.g. by order).
  2. Specimens are selected for individual DNA extraction, building a reference set. Ideally, one representative of each (morphological) species encountered at an Observatory is chosen, to build a reference library.
  3. Photographs of each specimen are maintained in a corresponding photo library, either from an individual image or taken from ensemble images with the Inselect software.
  4. Individual specimen DNA are used for shotgun sequencing or DNA barcoding; the remaining bulk is subjected to mixed shotgun sequencing or metabarcoding.
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© COPYRIGHT 2019 SITE100. ALL RIGHTS RESERVED.
  • SITE100
    • PROJECT OVERVIEW
    • PROJECT AIMS
  • METHODOLOGY
    • General workflow
    • Field protocols >
      • BUILDING A FLIGHT INTERCEPTION TRAP
    • Specimen processing
    • Soil extractions
  • STUDY SITES
    • Oriental >
      • MIZORAM STATE, INDIA
      • HONGKONG
      • THAILAND
      • BORNEO (DANUM VALLEY), SABAH, MALAYSIA
      • GAOLIGONG MOUNTAINS
    • Palaearctic >
      • QINGLING MOUNTAINS, CHINA
      • BOOKHAM COMMON, UK
      • EUROPEAN ISLANDS (IBIOGEN PROJECT)
      • SPAIN
      • SOUTHERN URALS
      • TIANMU MOUNTAINS
      • GUTIAN MOUNTAINS
    • Americas >
      • PANAMA: CERRO HOYA
      • SOUTHERN APPALACHIANS, USA
      • PANAMA: BARRO COLORADO ISLAND
      • PANAMA: SANTA FE
      • HONDURAS
      • HUATULCO, MEXICO
      • CHAMELA, MEXICO
      • FRENCH GUIANA
  • SITE100 SERVER
  • GET INVOLVED
    • Adding an observatory
    • Soil observatories
    • Local barcoding studies
    • Naturalists and community science
    • PhD students and postdocs
  • CONTACT
  • New Page