SITE-100
  • SITE100
    • PROJECT OVERVIEW
    • PROJECT AIMS
  • METHODOLOGY
    • General workflow
    • Field protocols >
      • BUILDING A FLIGHT INTERCEPTION TRAP
    • Specimen processing
    • Soil extractions
  • STUDY SITES
    • Oriental >
      • MIZORAM STATE, INDIA
      • HONGKONG
      • THAILAND
      • BORNEO (DANUM VALLEY), SABAH, MALAYSIA
      • GAOLIGONG MOUNTAINS
    • Palaearctic >
      • QINGLING MOUNTAINS, CHINA
      • BOOKHAM COMMON, UK
      • EUROPEAN ISLANDS (IBIOGEN PROJECT)
      • SPAIN
      • SOUTHERN URALS
      • TIANMU MOUNTAINS
      • GUTIAN MOUNTAINS
    • Americas >
      • PANAMA: CERRO HOYA
      • SOUTHERN APPALACHIANS, USA
      • PANAMA: BARRO COLORADO ISLAND
      • PANAMA: SANTA FE
      • HONDURAS
      • HUATULCO, MEXICO
      • CHAMELA, MEXICO
      • FRENCH GUIANA
  • SITE100 SERVER
  • GET INVOLVED
    • Adding an observatory
    • Soil observatories
    • Local barcoding studies
    • Naturalists and community science
    • PhD students and postdocs
  • CONTACT
  • New Page
Picture

Southern Appalachians, USA

Picture
Site name: Southern Appalachian Mountains, USA

Latitude/Longitude: 35.6, -82.4

Project lead: Michael S. Caterino, Clemson University Arthropod Collection (CUAC, South Carolina, USA).

Status of project: >300 litter samples from ~100 sites; sampling began in 2014 and is ongoing, adding samples primarily from higher elevation coniferous forests.

Available specimens and data:

(a) Samples: ~300 Berlesed samples, each with hundreds to thousands of specimens; Coleoptera largely removed and sorted to morphospecies; bulk residues remain in 100% ethanol in the CUAC.

(b) Reference specimen set: 380 morphospecies of Coleoptera have been recognized to date; vouchers are in CUAC (see Caterino et al. 2017). 

(c) DNA extractions: ~800 single specimen extractions of Coleoptera (largely of Carabidae: Anillini and Trechini; Staphylinidae: Dasycerus carolinensis; Curculionidae: Eurhoptus spp.); fifteen 96-well plate format extractions representing most other terrestrial arthropod groups. Additional plate extractions are being added frequently. Voucher images may be browsed on the Caterino Lab's Flickr site: 
https://www.flickr.com/photos/183480085@N02/

(d) DNA sequencing: Sanger sequenced COI, CAD, ITS2, KKV; Illumina sequenced COI (barcoding region) and 16S. 

(e) metabarcoding: COI and 16S for about 200 ‘miscellaneous arthropods’

(f) Sequence data:  (GenBank accession MH023427-MH023494, MH040342-MH040532, MH628652-MH628663, and MH638626-MH638629; MK834374-MK834510)


References:
  1. Anderson, R.S. and Caterino, M.S. 2018. A revision of the genus Eurhoptus LeConte 1876 (Curculionidae: Cryptorhynchinae) of America north of Mexico. ZooKeys 787: 37-80. (https://zookeys.pensoft.net/articles.php?id=26948)
  2. Caterino, M.S. and S.S. Langton-Myers. 2018. Long-term population persistence of flightless weevils (Eurhoptus pyriformis) across old- and second-growth forests patches in southern Appalachia. BMC Evolutionary Biology 18(165): 1-16. (doi.org/10.1186/s12862-018-1278-y)
  3. Caterino, M.S. and S.S. Langton-Myers. 2019. Intraspecific diversity and phylogeography in Southern Appalachian Dasycerus carolinensis Horn.Insect Systematics & Diversity 3(6): 1-12. (doi.org/10.1093/isd/ixz022)
  4. Caterino, M.S., Vásquez-Vélez, L.M., and S.S. Myers. 2017. On the conservation value of second-growth forests for leaf-litter inhabiting beetles. Insect Systematics & Diversity 1(1): 20-28. (https://academic.oup.com/isd/article/1/1/20/4563767)

Picture

Navigation

Home
Methodology
Study Sites
SITE100 Server
Get Involved

Support

Contact
© COPYRIGHT 2019 SITE100. ALL RIGHTS RESERVED.
  • SITE100
    • PROJECT OVERVIEW
    • PROJECT AIMS
  • METHODOLOGY
    • General workflow
    • Field protocols >
      • BUILDING A FLIGHT INTERCEPTION TRAP
    • Specimen processing
    • Soil extractions
  • STUDY SITES
    • Oriental >
      • MIZORAM STATE, INDIA
      • HONGKONG
      • THAILAND
      • BORNEO (DANUM VALLEY), SABAH, MALAYSIA
      • GAOLIGONG MOUNTAINS
    • Palaearctic >
      • QINGLING MOUNTAINS, CHINA
      • BOOKHAM COMMON, UK
      • EUROPEAN ISLANDS (IBIOGEN PROJECT)
      • SPAIN
      • SOUTHERN URALS
      • TIANMU MOUNTAINS
      • GUTIAN MOUNTAINS
    • Americas >
      • PANAMA: CERRO HOYA
      • SOUTHERN APPALACHIANS, USA
      • PANAMA: BARRO COLORADO ISLAND
      • PANAMA: SANTA FE
      • HONDURAS
      • HUATULCO, MEXICO
      • CHAMELA, MEXICO
      • FRENCH GUIANA
  • SITE100 SERVER
  • GET INVOLVED
    • Adding an observatory
    • Soil observatories
    • Local barcoding studies
    • Naturalists and community science
    • PhD students and postdocs
  • CONTACT
  • New Page