Southern Appalachians, USA
Site name: Southern Appalachian Mountains, USA
Latitude/Longitude: 35.6, -82.4
Project lead: Michael S. Caterino, Clemson University Arthropod Collection (CUAC, South Carolina, USA).
Status of project: >300 litter samples from ~100 sites; sampling began in 2014 and is ongoing, adding samples primarily from higher elevation coniferous forests.
Available specimens and data:
(a) Samples: ~300 Berlesed samples, each with hundreds to thousands of specimens; Coleoptera largely removed and sorted to morphospecies; bulk residues remain in 100% ethanol in the CUAC.
(b) Reference specimen set: 380 morphospecies of Coleoptera have been recognized to date; vouchers are in CUAC (see Caterino et al. 2017).
(c) DNA extractions: ~800 single specimen extractions of Coleoptera (largely of Carabidae: Anillini and Trechini; Staphylinidae: Dasycerus carolinensis; Curculionidae: Eurhoptus spp.); fifteen 96-well plate format extractions representing most other terrestrial arthropod groups. Additional plate extractions are being added frequently. Voucher images may be browsed on the Caterino Lab's Flickr site: https://www.flickr.com/photos/183480085@N02/
(d) DNA sequencing: Sanger sequenced COI, CAD, ITS2, KKV; Illumina sequenced COI (barcoding region) and 16S.
(e) metabarcoding: COI and 16S for about 200 ‘miscellaneous arthropods’
(f) Sequence data: (GenBank accession MH023427-MH023494, MH040342-MH040532, MH628652-MH628663, and MH638626-MH638629; MK834374-MK834510)
References:
Latitude/Longitude: 35.6, -82.4
Project lead: Michael S. Caterino, Clemson University Arthropod Collection (CUAC, South Carolina, USA).
Status of project: >300 litter samples from ~100 sites; sampling began in 2014 and is ongoing, adding samples primarily from higher elevation coniferous forests.
Available specimens and data:
(a) Samples: ~300 Berlesed samples, each with hundreds to thousands of specimens; Coleoptera largely removed and sorted to morphospecies; bulk residues remain in 100% ethanol in the CUAC.
(b) Reference specimen set: 380 morphospecies of Coleoptera have been recognized to date; vouchers are in CUAC (see Caterino et al. 2017).
(c) DNA extractions: ~800 single specimen extractions of Coleoptera (largely of Carabidae: Anillini and Trechini; Staphylinidae: Dasycerus carolinensis; Curculionidae: Eurhoptus spp.); fifteen 96-well plate format extractions representing most other terrestrial arthropod groups. Additional plate extractions are being added frequently. Voucher images may be browsed on the Caterino Lab's Flickr site: https://www.flickr.com/photos/183480085@N02/
(d) DNA sequencing: Sanger sequenced COI, CAD, ITS2, KKV; Illumina sequenced COI (barcoding region) and 16S.
(e) metabarcoding: COI and 16S for about 200 ‘miscellaneous arthropods’
(f) Sequence data: (GenBank accession MH023427-MH023494, MH040342-MH040532, MH628652-MH628663, and MH638626-MH638629; MK834374-MK834510)
References:
- Anderson, R.S. and Caterino, M.S. 2018. A revision of the genus Eurhoptus LeConte 1876 (Curculionidae: Cryptorhynchinae) of America north of Mexico. ZooKeys 787: 37-80. (https://zookeys.pensoft.net/articles.php?id=26948)
- Caterino, M.S. and S.S. Langton-Myers. 2018. Long-term population persistence of flightless weevils (Eurhoptus pyriformis) across old- and second-growth forests patches in southern Appalachia. BMC Evolutionary Biology 18(165): 1-16. (doi.org/10.1186/s12862-018-1278-y)
- Caterino, M.S. and S.S. Langton-Myers. 2019. Intraspecific diversity and phylogeography in Southern Appalachian Dasycerus carolinensis Horn.Insect Systematics & Diversity 3(6): 1-12. (doi.org/10.1093/isd/ixz022)
- Caterino, M.S., Vásquez-Vélez, L.M., and S.S. Myers. 2017. On the conservation value of second-growth forests for leaf-litter inhabiting beetles. Insect Systematics & Diversity 1(1): 20-28. (https://academic.oup.com/isd/article/1/1/20/4563767)
